flow cytometric data analysis software  (GraphPad Software Inc)

Journal: The Journal of Clinical Investigation

Article Title: Haploinsufficiency of immune checkpoint receptor CTLA4 induces a distinct neuroinflammatory disorder

doi: 10.1172/JCI135947

Figure Lengend Snippet: Flow cytometric data comparing patients with CTLA4h with active neuroinflammatory lesions on MRI (black), a prior history of neuroinflammatory lesions (green), and no history of inflammatory lesions (orange) on MRI with a cohort of healthy controls (NDs, white circles). All graphs depict values from blood (left half of each panel) and CSF (right half). (A) Total frequency of CD4+ and CD8+ T cells within total lymphocyte counts and the ratio of CD4+ to CD8+ T cells. (B) Percentage of memory T cell subtypes (CD45RA–CD27+) in CD4+ T cells and CD8+ T cells, and memory Tfh cells (CD45RA–CXCR5+) in CD4+ T cells (right). (C) Frequency of total B cells and memory B cell subtypes, including unswitched memory (IgD+CD27+) and switched memory (IgD–CD27+) B cells. For all flow cytometric data, the Mann-Whitney U test was performed for comparisons between ND and CTLA4 cohorts, within each compartment (i.e., peripheral blood and CSF). Significance was set at P < 0.05. Each flow cytometric experiment for lymphocyte subsets from peripheral blood that was paired with a CSF sample obtained at the same time represents a single experiment, given the nature of the specimen (CSF obtained by LP) and the limited amount of specimen that could be obtained, as well as the sensitivity for the timing of the procedure according to the clinical status and the treatment course of the patient.

Article Snippet: Statistical analysis of flow cytometric data was performed using GraphPad Prism (GraphPad Software).

Techniques: MANN-WHITNEY